Analysis of fecal populations of bifidobacteria and lactobacilli and investigation of the immunological responses of their human hosts to the predominant strains.
نویسندگان
چکیده
The bifidobacterial and lactobacillus populations of fecal samples collected from 10 human subjects were studied. The numbers of bifidobacteria were similar in the fecal samples of all of the subjects, but lactobacillus numbers varied, even between samples collected from the same individual. Analysis of the composition of the bacterial populations by ribotyping and pulsed-field gel electrophoresis to differentiate between strains showed that, at least for the numerically predominant strains, each subject harbored a unique collection of bifidobacteria and lactobacilli. Predominant bifidobacterial and lactobacillus strains detected in the feces of each subject were used in immunological assays (lymphocyte transformation, serum antibody titers) to determine the influence of the bacteria on the immune system of their host. Immunoglobulin G antibodies reactive with lactobacilli were detected at high concentrations; antibodies reactive with bifidobacteria were present at lower concentrations. The antibodies appeared to be genus specific rather than strain specific. The results of the study emphasized the complexity of the relationship that exists between the intestinal microflora and the human host.
منابع مشابه
Investigation of Acid and Bile Tolerance of Native Lactobacilli Isolated from Fecal Samples and Commercial Probiotics by Growth and Survival Studies
This study aimed at applying both growth and survival approaches to compare three native strains of lactobacilli, belonging to Lactobacillus plantarum, Lactobacillus rhamnosus and Lactobacillus acidophilus species, with two commercial probiotic strains in their tolerance to acid and bile. The association between the data obtained from the methods was studied. The results of the different metho...
متن کاملHow Probiotic Reduce Symptoms of Irritable Bowel Syndrome?
Introduction: Irritable bowel syndrome (IBS) is a common gastrointestinal disorder in children that may lead to anxiety, frequent physician visits and school absenteeism. The aim of this study is to reviewe effects of probiotic for irritable bowel syndrome. Materials and Methods: This study review articles about probiotic for irritable bowel syndrome in pubmed and google scholar. Results: M...
متن کاملCharacterization and Phylogenetic Analysis of Magnaporthe spp. strains on Various Hosts in Iran
Background: Populations of Magnaporthe, the causal agent of rice blast disease, are pathotypically and genetically diverse and therefore their interaction with different rice cultivars and also antagonistic microorganisms are very complicated. Objectives: The objectives of the present study were to characterize phylogenetic relationships of 114 native Magnaporthe strains, isolated from rice a...
متن کاملGenotypic and Phylogenic Analysis of Lactobacilli Producing Bacteriocin Isolated from Traditional Dairy Products and Food
Background & Objective: Lactic acid bacteria (LAB) are a group of Gram-positive, non-spore forming, cocci or rod shaped, catalase negative organisms, considered as Generally Recognized as Safe (GRAS) organisms. These bacteria are used for thousands of years for production of fermented foods because of their ability to produce desirable changes in taste, flavor and texture. Different antimicrobi...
متن کاملMolecular Identification of Giardia intestinalis and Blastocystis hominis Genotypes from Fecal Samples in Khuzestan Province, Iran
Background and purpose: Giardia and Blastocystis are common parasites of humans with a wide range of hosts. The aim of this study was to identify the genotypes of Giardia intestinalis and Blastocystis hominis in individuals attending Bavi health centers in Khuzestan province, Iran. Materials and methods: In this study, 30 positive stool samples of Giardia and Blastocystis were collected from i...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 63 9 شماره
صفحات -
تاریخ انتشار 1997